Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F17%3AE0106373" target="_blank" >RIV/00843989:_____/17:E0106373 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/15321819.2016.1233889" target="_blank" >http://dx.doi.org/10.1080/15321819.2016.1233889</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15321819.2016.1233889" target="_blank" >10.1080/15321819.2016.1233889</a>

Result language
angličtina
Original language name
Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method
Original language description
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using HevyliteTM antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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