Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite antibodies. comparison with the free light chain oligoclonal pattern

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F12%3A00102563" target="_blank" >RIV/00843989:_____/12:00102563 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/2045-8118-9-5" target="_blank" >http://dx.doi.org/10.1186/2045-8118-9-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/2045-8118-9-5" target="_blank" >10.1186/2045-8118-9-5</a>

Result language

angličtina

Original language name

Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite antibodies. comparison with the free light chain oligoclonal pattern

Original language description

Oligoclonal IgG bands in cerebrospinal fluid that are absent in serum indicate intrathecal IgG synthesis and are a sensitive marker of CNS inflammatory diseases, in particular multiple sclerosis. It may be of interest to determine whether these bands arepredominantly IgG? or IgG?. We have used Hevylite antibodies and developed a technique for detection of oligoclonal IgG? and IgG? bands by means of isoelectric focusing followed by immunoblotting. The same technique was used for oligoclonal free ? and free ? detection. Among several techniques tested, affinity immunoblotting appears to be the most sensitive; it can detect less than 1 ng of IgG? or IgG? paraprotein. We compared oligoclonal IgG profiles with those of oligoclonal IgG? and IgG?. There wasgood agreement concerning the presence or absence of intrathecal synthesis. We observed the ratios between oligoclonal IgG? and IgG? bands, and they did not always match the ratios between free ? and free ? bands. We were also able to det

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fluids and barriers of the CNS [online]

ISSN

2045-8118

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

5

UT code for WoS article

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EID of the result in the Scopus database

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