Robust induction of primordial germ cells of white rhinoceros on the brink of extinction.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F08568901%3A_____%2F22%3AN0000001" target="_blank" >RIV/08568901:_____/22:N0000001 - isvavai.cz</a>
Result on the web
<a href="https://www.science.org/doi/epdf/10.1126/sciadv.abp9683" target="_blank" >https://www.science.org/doi/epdf/10.1126/sciadv.abp9683</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.abp9683" target="_blank" >10.1126/sciadv.abp9683</a>
Alternative languages
Result language
angličtina
Original language name
Robust induction of primordial germ cells of white rhinoceros on the brink of extinction.
Original language description
In vitro gametogenesis, the process of generating gametes from pluripotent cells in culture, is a powerful tool for improving our understanding of germ cell development and an alternative source of gametes. Here, we induced primordial germ cell–like cells (PGCLCs) from pluripotent stem cells of the northern white rhinoceros (NWR), a species for which only two females remain, and southern white rhinoceros (SWR), the closest species to the NWR. PGCLC differentiation from SWR embryonic stem cells is highly reliant on bone morphogenetic protein and WNT signals. Genetic analysis revealed that SRY-box transcription factor 17 (SOX17) is essential for SWR-PGCLC induction. Under the defined condition, NWR induced pluripotent stem cells differentiated into PGCLCs. We also identified cell surface markers, CD9 and Integrin subunit alpha 6 (ITGA6), that enabled us to isolate PGCLCs without genetic alteration in pluripotent stem cells. This study provides a first step toward the production of NWR gametes in culture and understanding of the basic mechanism of primordial germ cell specification in a large animal.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science Advances
ISSN
2375-2548
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
49
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
1-19
UT code for WoS article
000913179000007
EID of the result in the Scopus database
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