Naive-like pluripotent to pave the way for saving the northern white rhinoceros from extinction.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F08568901%3A_____%2F22%3AN0000005" target="_blank" >RIV/08568901:_____/22:N0000005 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-022-07059-w#Abs1" target="_blank" >https://www.nature.com/articles/s41598-022-07059-w#Abs1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-022-07059-w" target="_blank" >10.1038/s41598-022-07059-w</a>

Result language

angličtina

Original language name

Naive-like pluripotent to pave the way for saving the northern white rhinoceros from extinction.

Original language description

The northern white rhinoceros (NWR) is probably the earth’s most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

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e-ISSN

2045-2322

Volume of the periodical

12

Issue of the periodical within the volume

3100

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

1-18

UT code for WoS article

000826474600008

EID of the result in the Scopus database

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