In Vitro Proliferation of Newly Bred Czech Pear Cultivars

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F09%3A%230000105" target="_blank" >RIV/25271121:_____/09:#0000105 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In Vitro Proliferation of Newly Bred Czech Pear Cultivars
Original language description
The objective of this study was to develop a method for rapid in vitro shoot multiplication of four newly bred Czech pear cultivars (Bohemica, Dicolor, Elektra and Erika). Six proliferation MS media containing different concentrations of cytokinins BAP (6-benzylaminopurine), TDZ (thidiazuron) or 2iP (6-(g,g-dimethylallylamino) purine) were tested. Selected pear cultivars were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Of the 80 shoot tips taken only 3 explants of Bohemica, 2 explants of Erika and 1 explant of Dicolor were visibly contaminated. The highest proliferation rate was obtained for pear cultivar Elektra that produced 4.1 shoots (longer than 10 mm) on MS medium containing 1mg/L TDZ. The lowest proliferation rate was obtained for cultivar Dicolor, which did not multiply at all on MS medium containing the lowest concentration of BAP 1 mg/L.
Czech name
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Czech description
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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