Micropropagation of old pear cultivars

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F12%3A%230000504" target="_blank" >RIV/25271121:_____/12:#0000504 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Micropropagation of old pear cultivars
Original language description
The goal of this study was to develop a method for rapid in vitro shoot multiplication of five older pear cultivars and landraces ('Beurre de Merode', 'Grise Bonne', 'Holzfarbige Butterbirne', 'Jakubka ceska', 'Solanka'). Six proliferation MS media containing different concentrations of cytokinins BAP (6-benzyl-aminopurine), TDZ (thidiazuron) or 2iP 6-(gamma,gamma-Dimethylallylamino) purine were tested. For the five genotypes, the effect of three growth regulators on proliferation, callus formation andshoot morphology is shown. Selected pear cultivars were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Of the 100 shoot tips taken only 1 explant of 'Holzfarbige Butterbirne' and 6 explants of 'Solanka' were visibly contaminated with microorganisms. The number of newly formed shoots varied with the cultivar and concentration of cytokinins. Generally, the highest proliferation rate was obtained for pear cultivar 'Grise Bo
Czech name
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Czech description
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Project
<a href="/en/project/QH91148" target="_blank" >QH91148: Research on resistance of pome fruit landraces to fireblight and improving of testing with use of biotechnological methods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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