Protocol for in vitro shoot multiplication of ancient pear cultivars and landraces

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000008" target="_blank" >RIV/25271121:_____/21:N0000008 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 " target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 </a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1303.15 " target="_blank" >10.17660/ActaHortic.2021.1303.15 </a>

Result language

angličtina

Original language name

Protocol for in vitro shoot multiplication of ancient pear cultivars and landraces

Original language description

In 2016, a project was started to preserve the diversity of older pear cultivars grown in the Czech Republic. The purpose of this study was to develop an efficient in vitro system for rapid propagation of pear explants as initial plant material for cryopreservation experiments. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks. Selected pear genotypes ‘Avranches’, ‘Jakobibirne’, ‘Marillat’, ‘Flamandka’ were successfully established in vitro using 0.15 % mercuric chloride as disinfectant. Six MS-based media containing 1, 2 or 4 mg L-1 zeatin or 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine) were used for multiplication experiments. Multiplication rate was defined as the number of newly formed shoots (10 mm) per initial shoot tip after four weeks of culture. Cultivars in the study differed in their proliferation and development potential in MS medium according to hormone level between 1.0 and 3.8. Generally, the highest proliferation rate (3.8) in our experiments was obtained for cultivar ‘Marillat’ on MS medium with 2 mg L-1 BAP. Sufficient multiplication at a rate of 3.1 was obtained only for ‘Marillat’ on a medium with the highest concentration cytokinin zeatin of 4 mg L-1. Results obtained in our study confirmed preliminary findings that BAP was an important plant growth regulator for proliferation and growth in pear micropropagation. During the in vitro multiplication phase, for all tested cultivars, no morphological abnormalities such as excessive callus formation, hyperhydricity or production of abnormally narrow leaves were noted.

Czech name

—

Czech description

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Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/LO1608" target="_blank" >LO1608: Research Pomological Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

—

ISSN

0567-7572

e-ISSN

—

Number of pages

6

Pages from-to

101-106

Publisher name

ISHS

Place of publication

Leuven

Event location

Montevideo; Uruguay

Event date

Dec 3, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000706559200015