Development of a system for testing apple resistence to Erwinia amylovora using in vitro culture techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F15%3A%230000592" target="_blank" >RIV/25271121:_____/15:#0000592 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of a system for testing apple resistence to Erwinia amylovora using in vitro culture techniques

Original language description

The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility of two cultivars due to the different speed of fire blight symptom development.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

1-5

UT code for WoS article

000351997700001

EID of the result in the Scopus database

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