Development of tissue culture system for pear cultivars

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000034" target="_blank" >RIV/25271121:_____/17:N0000034 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.21" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1155.21</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.21" target="_blank" >10.17660/ActaHortic.2017.1155.21</a>

Result language

angličtina

Original language name

Development of tissue culture system for pear cultivars

Original language description

The pear is one of the most economically important fruit crops in the temperate climatic zone. Concerning the Czech Republic, there were 790 ha of commercial pear plantations in 2014. In 2012, a project has been started to test the resistance of pear landraces and older cultivars grown on the territory of the Czech Republic to fire blight in in vitro culture and field conditions. A key part of the project was also testing the applicability of different in vitro culture media and methods of artificial inoculation in in vitro culture conditions. In the present study, successful micropropagation, which can be carried out during the whole year, provided an alternative method to produce stock material of particular cultivars for experiments with artificial inoculation. The donor shoots were obtained in March from mature trees grown at the Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd., Department of Genebanks. Selected genotypes were successfully established in vitro using 0.15% mercuric chloride as disinfection solution. Shoots developed were subcultured onto fresh MS media for six consecutive 4-week passages. Five MS media containing 1, Z and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) were tested. Selected pear cultivars differed in their multiplication and development potential on tested media. Multiplication coefficients and statistical evaluation are stated in the paper. The effect of selected growth regulators on callus formation and shoot morphology is also presented. In conclusion, obtained data show that in vitro propagation of used pear cultivars can be accomplished in amount sufficient for artificial inoculation studies. We obtained multiplication coefficient higher than 3.0 for all tested pear genotypes. Generally, the highest proliferation rate was obtained for pear cultivar 'Bitterbirne Lukas' that produced 4.9 in vitro shoots on MS medium containing 4 mg L-1 BAP. The physiological mechanisms that determine the need for growth regulators during the different stages ofin vitro development are currently being investigated.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QJ1210184" target="_blank" >QJ1210184: The utilization of biotechnological methods for more effective testing of pome fruit resistance to fire blight (bacterium Erwinia amylovora)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

978-94-6261-151-1

ISSN

0567-7572

e-ISSN

2406-6168

Number of pages

4

Pages from-to

157-160

Publisher name

ISHS

Place of publication

Leuven

Event location

Sanremo, Italy

Event date

Apr 19, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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