Determination of major phenolic compounds in apples: Part I-Optimization of high-performance liquid chromatography separation with diode array detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F18%3AN0000010" target="_blank" >RIV/25271121:_____/18:N0000010 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/18:10377488
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201800302" target="_blank" >http://dx.doi.org/10.1002/jssc.201800302</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201800302" target="_blank" >10.1002/jssc.201800302</a>
Alternative languages
Result language
angličtina
Original language name
Determination of major phenolic compounds in apples: Part I-Optimization of high-performance liquid chromatography separation with diode array detection
Original language description
The separation of seven phenolic compounds including gallic acid, chlorogenic acid, epicatechin, quercitrin, rutin, phloridzin, and phloretin present in apple peel and pulp and differing in elution properties has been optimized using high-performance liquid chromatography with diode array detection. Several stationary phases were tested to achieve the efficient separation of phenolic compounds in fruit extracts and C18 was found to be the most efficient. Core-shell and fully porous C18 packings were assessed with respect to the complex composition of the fruit extracts. The developed high-performance liquid chromatography method comprised gradient elution in which mobile phase A was water at pH 2.8 adjusted with acetic acid and B was acetonitrile. The gradient shape was the following: 0 min 95% A/5% B, 2.5 min 85% A/15% B, 12 min 50% A/50% B, 15 min 95% A/5% B. The flow rate was 1 mL/min, injection volume 10 mu L, and UV detection at 255, 280, 320, and 365 nm was applied. Our method was validated for both C18 core-shell and fully porous packings. The resolution 6.2-14.8, symmetry 0.99-1.34, peak capacity 18-60, peak area repeatability 0.45-1.00% relative standard deviation, calibration range 0.125-5 mg/mL (0.2510 mg/mL for chlorogenic acid and rutin), correlation coefficients of calibration curve 0.9976-0.9997, and accuracy evaluated as recovery 95.56-107.54% were determined for the core-shell column.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
15
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
3042-3050
UT code for WoS article
000440842600004
EID of the result in the Scopus database
2-s2.0-85051193553