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Slow growth in vitro conservation of fruit crops

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000093" target="_blank" >RIV/25271121:_____/19:N0000093 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1234.15</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >10.17660/ActaHortic.2019.1234.15</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Slow growth in vitro conservation of fruit crops

  • Original language description

    Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd. holds a fruit germplasm of about 2500 accessions in field collections. Unfortunately trees in these collections are at permanent risk from environmental conditions, pests and diseases. Germplasm preservation of vegetatively propagated fruit species in field collections may be supplemented using biotechnological methods. In vitro storage under slow-growth conditions is one of these techniques. The experiment with medium term storage of in vitro cultures of strawberry, apple, pear and sweet cherry in low above zero temperature 4.0 °C was used in RBIP Holovousy Ltd. as a duplicate method to field collections. In vitro cultures were established on MS medium supplemented with 1 mg · L-1 6-benzylaminopurine (BAP) in aluminium-capped 100 ml Erlenmeyer flasks with 5 explants/25 ml of medium. The experiment was successful and viable shoot tips were obtained at the end of storage period. The best overall results were obtained with sweet cherry rootstock genotype P-HL-A, where 92.0 % explants survived without an intervening subculture after seven months. On contrast, the lowest survival was observed with sweet cherry P-HL-C, where only 2.0 % of shoots survived 7 months from the beginning of experiment. Remaining genotypes of fruit species used in the experiment had intermediate results. The storage of in vitro cultures at 4 °C appears to be promising technique as a duplicate medium-term conservation method for fruit germplasm, which can considerably reduce labor and material costs. Moreover in vitro collections are year-long accessible form of germplasm for distribution among germplasm curators and user community.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    40401 - Agricultural biotechnology and food biotechnology

Result continuities

  • Project

    <a href="/en/project/LO1608" target="_blank" >LO1608: Research Pomological Centre</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Acta Horticulturae

  • ISBN

    9789462612310

  • ISSN

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    119-124

  • Publisher name

    ISHS

  • Place of publication

    Leuven

  • Event location

    Bangkok; Thailand

  • Event date

    Mar 26, 2018

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article