Slow growth in vitro conservation of fruit crops
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000093" target="_blank" >RIV/25271121:_____/19:N0000093 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1234.15</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.15" target="_blank" >10.17660/ActaHortic.2019.1234.15</a>
Alternative languages
Result language
angličtina
Original language name
Slow growth in vitro conservation of fruit crops
Original language description
Research and Breeding Institute of Pomology (RBIP) Holovousy Ltd. holds a fruit germplasm of about 2500 accessions in field collections. Unfortunately trees in these collections are at permanent risk from environmental conditions, pests and diseases. Germplasm preservation of vegetatively propagated fruit species in field collections may be supplemented using biotechnological methods. In vitro storage under slow-growth conditions is one of these techniques. The experiment with medium term storage of in vitro cultures of strawberry, apple, pear and sweet cherry in low above zero temperature 4.0 °C was used in RBIP Holovousy Ltd. as a duplicate method to field collections. In vitro cultures were established on MS medium supplemented with 1 mg · L-1 6-benzylaminopurine (BAP) in aluminium-capped 100 ml Erlenmeyer flasks with 5 explants/25 ml of medium. The experiment was successful and viable shoot tips were obtained at the end of storage period. The best overall results were obtained with sweet cherry rootstock genotype P-HL-A, where 92.0 % explants survived without an intervening subculture after seven months. On contrast, the lowest survival was observed with sweet cherry P-HL-C, where only 2.0 % of shoots survived 7 months from the beginning of experiment. Remaining genotypes of fruit species used in the experiment had intermediate results. The storage of in vitro cultures at 4 °C appears to be promising technique as a duplicate medium-term conservation method for fruit germplasm, which can considerably reduce labor and material costs. Moreover in vitro collections are year-long accessible form of germplasm for distribution among germplasm curators and user community.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology
Result continuities
Project
<a href="/en/project/LO1608" target="_blank" >LO1608: Research Pomological Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Acta Horticulturae
ISBN
9789462612310
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
119-124
Publisher name
ISHS
Place of publication
Leuven
Event location
Bangkok; Thailand
Event date
Mar 26, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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