A new allelic discrimination real-time PCR assay for PavMYB10.1 genotyping to predict sweet cherry fruit colour: a comparison with the Pav-Rf-SSR assay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000158" target="_blank" >RIV/25271121:_____/21:N0000158 - isvavai.cz</a>

Result on the web

<a href="https://www.tandfonline.com/doi/full/10.1080/14620316.2020.1837021" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/14620316.2020.1837021</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/14620316.2020.1837021 " target="_blank" >10.1080/14620316.2020.1837021 </a>

Result language

angličtina

Original language name

A new allelic discrimination real-time PCR assay for PavMYB10.1 genotyping to predict sweet cherry fruit colour: a comparison with the Pav-Rf-SSR assay

Original language description

Fruit skin colour in cherries is one of the most important market characteristics with customer preferences varying across cultures and countries. Based on skin colour, cherries can be divided into three market classes: yellow, blush, and mahogany. The PavMYB10.1 gene has been recently shown to be a key player in fruit colouration, and three alleles (PavMYB10.1a [WT (5A)]; PavMYB10.1b [Mut(4A)]; and PavMYB10.1c [InDel]) have been associated with cherry skin colour. In the present study, an allelic discrimination real-time PCR assay was developed for marker-assisted selection (MAS). The Pav-Rf-SSR assay that had previously been described for colour prediction was also employed for comparison. Both assays were used to screen 302 phenotyped accessions in a germplasm collection. The real-time PCR assay reliably discriminated all six PavMYB10.1 genotypes, and accurately predicted skin colour in all PavMYB10.1 homozygous genotypes; however, for heterozygotes, the assay was uninformative. As for the Pav-Rf-SSR assay, only two out of six observed alleles were informative, and only for mahogany market class. Using combined approach, fruit skin colour could be successfully predicted in 67.9% of all germplasm samples. The real-time PCR assay for PavMYB10.1 genotyping thus represents suitable tool for laboratories interested in MAS for skin colour in sweet cherries.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40105 - Horticulture, viticulture

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Horticultural Science and Biotechnology

ISSN

1462-0316

e-ISSN

—

Volume of the periodical

96

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

260-269

UT code for WoS article

000589817500001

EID of the result in the Scopus database

2-s2.0-85094648371