In vitro micropropagation of sweet cherry cultivars

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F25%3AN0000045" target="_blank" >RIV/25271121:_____/25:N0000045 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In vitro micropropagation of sweet cherry cultivars

Original language description

Sweet cherry (Prunus avium L.) is an important fruit tree valuable for its nutritional and tasty fruits. In vitro micropropagation methods of sweet cherry can be applied in tree propagation, for experimental studies, cryotherapy, preservation or for transformation methods. The objective of this study was to compare two types of culture media and its ability to increase the bud survival rate of sweet cherry cultivars. A bud was extirpated from a 2-year-old sweet cherry shoot and cleaned from outer and inner scales. The bud was washed in sterile distilled water and sterilized by a sodium dichloroisocyanurate solution. After the sterilization the bud was washed again by sterile water, blotted dry and cultivated either on QL-1 or BAP 200 culture media for 2 months with set cultivation conditions. Buds were subcultured after 4 weeks of cultivation. Increased bud survival rate for all tested cultivars was detected when cultured on QL-1 medium compared to cultivation on BAP 200 medium. The survival rate of HL 10072 buds was significantly higher on QL-1 medium than on BAP 200. It was also the highest survival rate of all tested cultivars. Cultivars ´Tamara´, ´Bing´ and hybrid ´HL 10072´ had higher survival rates. Generally lower survival rates were detected for ´Kordia´ and HL 16521. Study demonstrated that QL-1 medium is able to increase bud survivals rate. Composition of medium could be more suitable for sweet cherry propagation. Some cultivars are more recalcitrant to in vitro cultivation than others. Successful introduction of certain cultivar plant material into in vitro conditions often depends on physiological needs of cultivar. The result was published in Book of Abstracts for 24th International Workshop for Young Scientists “BioPhys Spring 2025”, p. 33-34, ISBN 978-83-89969-94-1, 29th-30th May 2025, Prague, Czech Republic under auspices of Ministry of the Enviroment of the Czech Republic and Czech University of Life Sciences Prague.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů