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Genetic diversity of Pyrenophora teres isolates as detected by AFLP analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F05%3A%230000032" target="_blank" >RIV/25328859:_____/05:#0000032 - isvavai.cz</a>

  • Alternative codes found

    RIV/00027006:_____/05:219

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Genetic diversity of Pyrenophora teres isolates as detected by AFLP analysis

  • Original language description

    Amplified fragment length polymorphism (AFLP) analysis has been used to analyse mainly 83 Czech isolates of Pyrenophora teres, P. graminea, P. tritici-repentis and Helminthosporium sativum. Each species had distinct AFLP profiles. Using 19 primer combinations 948 polymorphic bands were detected. All main clusters in dendrogram correspond to the studied species. Even the two forms of P. teres-P. teres f. teres (PTT) and P. teres f. maculata (PTM) - formed different clusters. Genetic diversity, with regard to the locality and the year of the sample's collection, was analysed separately within the AFLP-based dendrogram cluster of PTT and PTM. Unweighted pair-group method (UPGMA) analysis of the 37 isolates of PTT and 30 isolates of PTM, using 469 polymorphic bands, showed that the variability seemed to be influenced more by the year of sampling than by the geographic origin of the isolate.

  • Czech name

    Genetická diverzita izolátů Pyrenophora teres zjištěná analýzou AFLP

  • Czech description

    K analýze 83 českých izolátů Pyrenophora teres, P. graminea, P. tritici-repentis a Helminthosporium sativum byla použita metoda AFLP. Každý druh se vyznačoval odlišným profilem AFLP. Pomocí 19 kombinací primerů bylo detekováno 948 polymorfních pruhů. Všechny hlavní klastry v dendrogramu odpovídají sledovaným druhům. Dokonce obě formy P. teres - P. teres f. teres (PTT) a P. teres f. maculata (PTM) ? vytvářely odlišné klastry. S ohledem na lokalitu a rok sběru vzorku byla genetická diverzita analyzována odděleně v rámci klastru dendrogramu PTT a PTM na základě AFLP. Analýza nevážené párové metody (UPGMA) u 37 izolátů PTT a 30 izolátů PTM, s využitím 469 polymorfních pruhů, prokázala, že variabilita byla více ovlivněna rokem odebrání vzorku než geografickým původem izolátu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/QC1361" target="_blank" >QC1361: Developent of a technique for the quantification of fungal pathogens based on Real-Time PCR approach.</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Phytopathology

  • ISSN

    0931-1785

  • e-ISSN

  • Volume of the periodical

    153

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    10

  • Pages from-to

    569-578

  • UT code for WoS article

  • EID of the result in the Scopus database