THE POSSIBILITIES FOR IDENTIFICATION AND CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CULTIVARS USING MICROSATELLITE MARKERS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F15%3A%230001417" target="_blank" >RIV/26296080:_____/15:#0001417 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

THE POSSIBILITIES FOR IDENTIFICATION AND CHARACTERIZATION OF ALFALFA (MEDICAGO SATIVA L.) CULTIVARS USING MICROSATELLITE MARKERS

Original language description

THE AIM OF THIS STUDY WAS TO ESTABLISH POSSIBILITIES OF CHOSEN SSR MARKERS FOR THE IDENTIFICATION AND DIFFERENTIATION A SET OF 37 ALFALFA CULTIVARS (MEDICAGO SATIVA L.) AND ALSO THE UTILIZATION THESE SSR MARKERS TO DETECT GENETIC DIVERSITY AMONG ALFALFA CULTIVARS. A TOTAL OF 5 SSR MARKERS WERE SELECTED FROM PREVIOUS STUDIES BASED ON THEIR HIGH POLYMORPHISM IDENTIFICATION CONTENT (PIC). ALL USED MARKERS WERE POLYMORPHIC AND PRODUCED REPRODUCIBLE, CONSISTENT AND EASILY SCORABLE DATA. THESE FIVE PRIMER PAIRS AMPLIFIED ALTOGETHER 18 DIFFERENT POLYMORPHIC ALLELES WITH AN AVERAGE NUMBER OF 3.6 ALLELES PER LOCUS. THE NUMBER OF ALLELES RANGED FROM 3 (FMT13) TO 6 (AFCTT1). THE EXPECTED HETEROZYGOSITY (HE) AND POLYMORPHIC INFORMATION CONTENT (PIC) OF THE TESTED SSR MARKERS WERE CALCULATED. THE HE OF THE TESTED SSR MARKERS RANGED FROM 0.5737 (FMT13) TO 0.7267 (AFCTT1) WITH AN AVERAGE OF 0.6297. THE PIC RANGED FROM 0.4921 (MTIC430) TO 0.6788 (AFCTT1) WITH AN AVERAGE OF 0.5593. THE DENDROGRAM BASED ON HIERARCHICAL CLUSTER ANALYSIS USING UPGMA ALGORITHM FOR THE DETECTION OF GENETIC DIVERSITY WAS CONSTRUCTED. THE CULTIVARS WERE GROUPED INTO THREE MAIN CLUSTERS. IT WAS NOT POSSIBLE TO DISTINGUISH CULTIVARS KOLY (YEM) & REMANY (YEM) AND KM - MARARTON (HUN) & CRENO (DNK) FROM EACH OTHER, RESPECTIVELY.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

GE - Plant cultivation

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

SEED AND SEEDLINGS

ISBN

978-80-213-2544-9

ISSN

—

e-ISSN

—

Number of pages

6

Pages from-to

120-125

Publisher name

CZU Praha

Place of publication

Praha

Event location

Praha

Event date

—

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

000357378800034