MALDI-TOF MS identification of spoilage and food-borne bacteria

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F16%3AN0000034" target="_blank" >RIV/26722861:_____/16:N0000034 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/16:43903139

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

MALDI-TOF MS identification of spoilage and food-borne bacteria

Original language description

The fast and reliable identification and typing of spoilage and food-borne microorganisms belong to the basic challenges in food microbiology. Methods testing physiological and biochemical features or analysing DNA are more or less time and work demanding. Furthemore, bacteria phylogenetic groups differ in the applied specific method protocols. In comparison, MALDI-TOF MS analysis of bacterial protein profiles provides a very rapid alternative, easy to perform and widely applicable. In this study we concern two parts of interest. Firstly we identified 320 isolates of spoilage and food-borne bacteria from different sources by MALDI-TOF MS (using Bruker Autoflex Speed and Biotyper 3.1) and by biochemical or species-specific PCR methods, or 16S rRNA sequencing in discordant results. This comparison allowed to detect possible problematic species or genera as e.g. biochemically atypical strains of Raoultella ornithinolytica. The different clustering algorithms were designed and tested for the species discrimination within such some Pseudomonas, Enterobacter cloacae and Bacillus species group. Secondly we tested the influence of different diagnostic-selective media, the temperatures of cultivation and storage, and the sample preparation method on the robustness of MALDI-TOF MS identification of Enterobacteriaceae. The sample preparation method was found to be the most important factor for reliable identification in case of prolonged storage of cultures grown diagnostic-selective media.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/QJ1210300" target="_blank" >QJ1210300: Protection systems of quality and safety of dairy products by means of suitable methods applicable in practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů