The occurrence of common species of the opportunistic fungal pathogens in dairies and bioptic samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F19%3AN0000062" target="_blank" >RIV/26722861:_____/19:N0000062 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The occurrence of common species of the opportunistic fungal pathogens in dairies and bioptic samples

Original language description

PP prezentace and Abstract The 2019 ICFM Conference on “Food- and Airborne Fungi – Challenges for Food Safety and Supply” Freising, Germany on June 03 - 05, 2019, p 5 – 6. The opportunistic fungal pathogens (OFP) are represented by fungal species that follow a wide spectrum of life strategies and inhabit various ecological niches. The OFP in dairy operations causes severe problems in spite of massive preventive measures. Contamination of products results in economic loss but also pose a health risk for workers and consumers. The objectives of this study were 1. To survey the occurrence of OFP in the environment and in products in HCCP dairies and family farm dairies and 2. To evaluate the OFP as causal agents of mycoses. The fungal contaminants were isolated and identified from the environment concerning their adaptability to the extreme conditions in dairy operations such as salt bath, pasteurization, temperature, sanitation. The microbial and fungal diversity of milk products from pasteurised and non-pasteurised milk were compared. The fungal isolates were analysed by using molecular methods and sequencing combined with classical microbiological methods. The anonymous bioptic samples were analysed based on the type of immunodeficiency and disease etiology to obtain the current status about the pathogenicity of OFP for immunocompromised individuals. NGS methods and DNA barcoding were further analyzed these samples to assign individual OFPs to the relevant pathological findings. The results showed that many OFP identified in pulmonary, dermatological and colon bioptic samples such as Aspergillus nigricans, Cladosporium spp., Alternaria spp., Fusarium spp. are also representatives of dairy contaminants. These species have a high adaptability and resistance to the extreme environment, antibiotics, and sanitation. The fungal diversity between HACCP dairies and farm dairies was found to be significantly different. The prevalence of Penicillium-species and yeast contaminants was found in farm samples whereas the occurrence of OFP contaminants resistant to sanitary chemicals was noted in HACCP dairies first at all.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/QK1910036" target="_blank" >QK1910036: Utilization of various metabolites of wild strains of lactic acid bacteria and yeasts to suppress the occurrence of undesirable fungal contaminants and to prolong the shelf life of dairy and bakery products.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů