Characterization of genetic diversity and structure analysis within pea (Pisum sativum L.ú germplasm at agritec LTd. collection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F07%3A%230000004" target="_blank" >RIV/26784246:_____/07:#0000004 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Characterization of genetic diversity and structure analysis within pea (Pisum sativum L.ú germplasm at agritec LTd. collection
Original language description
The 164 accessions representing Czech and Slovak varieties bred over last 50 years were selected for evaluation of genetic diversity in this study. These were characterized by 42 morphological descriptors in two growth seasons, and DNA of bulk made from10 plants per accession was investigated by several molecular markers, including microsatellite SSR and retrotransposon-based markers. The emphasis was put on easily scorable co-dominat RBIP (Retrotransposon-Based Insertion Polymorphism) markers .Molecular data were subjected to genetic distance analysis by NTSYS-pc software. Calculated genetic distance of investigated set ranged from 0.5 to 0.96 of Jaccard Coefficient. Cluster analysis by Ward method has yielded 6 to 7 clusters both for SSR and RBIP data. Euclidean distance clustering of morphological characters structured set into 5 to 7 clusters. To enable integration and evaluation of all data in one analysis, Bayesian method using BAPS software was applied.
Czech name
Charakterizace genetické diverzity kolekce hrachu
Czech description
164 položek hrachu vybraných z 1 244 položek současně uložených v kolekci Agritec v Šumperku bylo charakterizováno ve dvou vegetačních obdobích pomocí 42 ,morfologických deskriptorů, 12 vybraných mikrosatelitních markerů, 25 retrotranspsonových markerů ve formátu RBIP a dvou IRAP, což poskytlo celkem 65 molekulárních genetických dat pro každou položku. Shluková analýza Wardovou metodou identifikovala 6-7 shluků, Euklidovská analýza morfologických dat 5-7 shluků. K umožnění integrace všech těchto dat bylvyužit Bayesiánský přístup, který s vysokou hladinou pravděpodobnosti určil 3 až 6 shluků. Byla prověděna rovněž analýza PCA.
Classification
Type
D - Article in proceedings
CEP classification
GE - Plant cultivation
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Plant Genetic Resources and their Exploitation in the Plant Breeding for Food and Agriculture
ISBN
978-80-88872-63-4
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
54-55
Publisher name
PNprint s.r.o.
Place of publication
Piešťany
Event location
Piešťany, Slovensko
Event date
Jan 1, 2007
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—