A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F13%3A%230000761" target="_blank" >RIV/26784246:_____/13:#0000761 - isvavai.cz</a>

Result on the web

<a href="http://acta.mendelu.cz/pdf/actaun201361061785.pdf" target="_blank" >http://acta.mendelu.cz/pdf/actaun201361061785.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201361061785" target="_blank" >10.11118/actaun201361061785</a>

Result language

angličtina

Original language name

A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

Original language description

Ther was developed a new more rapid and reliable diagnostic method working on the RT-PCR platform for monitoring of MRSA/S. aureus. PCR of the S. aureus specific nuc gene sequence and the mecA gene sequence was utilised for this purpose. A collection often S. aureus/MRSA reference strains, fifteen genetically related non S. aureus reference strains and fifty-six environmental samples was employed for estimation of the assay performance and parameters. The classic selective cultivation approach with thebiochemical test and agar disk diffusion test was accepted as reference diagnostic method. Our method featured with 100% sensitivity in comparison to reference method. The limit of detection allowed to identify from tens to hundreds copies of S. aureus/MRSA genome. Further, the RT-PCR assay featured with 100% inclusivity and 95% exclusivity at Cq value below 30. We consider our method as ideal for testing of individual suspected colonies, when the results can be acquired in less than 1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/QJ1210284" target="_blank" >QJ1210284: Implementation of methods for MRSA detection in meat of food animals and methods for effective measures against their spread in the food chain.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Volume of the periodical

LXI

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

1785-1792

UT code for WoS article

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EID of the result in the Scopus database

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