The real-time PCR assay for rapid identification of MRSA strains in cow milk

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F12%3A%230000558" target="_blank" >RIV/49608851:_____/12:#0000558 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The real-time PCR assay for rapid identification of MRSA strains in cow milk
Original language description
The aim of our work was to provide a new, high-throughput and reliable method intended for rapid identification of MRSA in raw cow milk. The duplex real-time PCR assay working with the dual-labelled hydrolysis probes was developed. The sequence specificity of the nuc gene was utilized to design S. aureus specific assay. Detection of the methicillin resistance was based on the mecA gene sequence identifying. A collection of twenty S. aureus/ MRSA reference strains and twenty non - S. aureus strains inclusive Staphylococcus spp. with potential occurrence in milk was analysed to validate the parameters of the method. The incubation of artificially contaminated milk samples in solution of Bird-Parker medium was employed as a selective pre-enrichment step.The PCR assay featured with robust, specific and reproducible amplification. The exclusivity and inclusivity was almost absolute, the limit of the detection was estimated to be tens of copies of S. aureus genome. Our method could be effec
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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