Comparison of the performance of two different newly designed real-time PCR assays applicable for S. aureus/ MRSA identification

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F12%3A%230000614" target="_blank" >RIV/26788462:_____/12:#0000614 - isvavai.cz</a>
Alternative codes found
RIV/49608851:_____/12:#0000560
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Comparison of the performance of two different newly designed real-time PCR assays applicable for S. aureus/ MRSA identification
Original language description
The environment of farm animals was identified as an important source of pathogenic S. aureus strains including the antibiotic-resistant forms. These could contaminate the food chain or infect the humans. In order to improve the quality of existing microbial diagnostic tools, we designed two real-time PCR assays for identification of Staphylococcus aureus/ MRSA strains. The methods worked with different platform of PCR product detection: SYBR green dye and dual-labelled hydrolysis (TaqMan) probes. We compared the performance of the both assays with regard to its practical application. The both PCR assays featured with reproducible and robust amplification. The limit of the detection between the assays differed significantly. The SYBR green assay enabled detection of tens of S. aureus genome copies unlike hundreds of genome copies at TaqMan assay. A group of thirty reference strains of S. aureus/ MRSA and twenty-eight strains of different Staphylococcus spp. were analysed using the opti
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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