Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein ?-domain as a fusion to ?-glucuronidase gene protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F13%3A%230000793" target="_blank" >RIV/26784246:_____/13:#0000793 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s11240-012-0239-1" target="_blank" >http://link.springer.com/article/10.1007/s11240-012-0239-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>
Alternative languages
Result language
angličtina
Original language name
Enhanced accumulation of Cadmium in Linum ussitatissimum L. plants due to overproduction of metallothionein ?-domain as a fusion to ?-glucuronidase gene protein
Original language description
In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed. When tested in soils amended with Cd at 20 and 360 mg kg-1, the mature ?MT1a::gus plants accumulated moreCd than parental AGT 917: the stem Cd concentrations in the best performing ?MT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of ?MT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15mg Cd l-1, indicating that ?MT1a::gus did confer higher Cd tolerance to engineered plant. Overproduction of metal-binding peptides thus appears to be a viable strategy for the production of L. usitatissimum with improved phytoremediation
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell Tissue and Organ Culture
ISSN
1573-5044
e-ISSN
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Volume of the periodical
2013
Issue of the periodical within the volume
112
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
321-330
UT code for WoS article
000315277300006
EID of the result in the Scopus database
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