Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33146005" target="_blank" >RIV/61989592:15310/13:33146005 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/13:43895482

Result on the web

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >http://dx.doi.org/10.1007/s11240-012-0239-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>

Result language

angličtina

Original language name

Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein.

Original language description

As most cultivars of flax and linseed (Linum usitatissimum L.) are capable of accumulating cadmium (Cd), they are suitable candidates for phytoextraction of the metal from contaminated soils. In an attempt to enhance the phytoextraction capacity of L. usitatissimum through overproduction of an efficient heterologous Cd-binding peptide, we engineered linseed breeding line AGT 917 to constitutively express genetic fusion of a-domain of mammalian metallothionein 1a (aMT1a) and b-glucuronidase gus gene under the control of CaMV 35S promoter. An improved transformation protocol was developed, which involved cocultivation of AGT 917 hypocotyl segments with partially removed epidermis with Agrobacterium suspension for 10 min in the presence of 200 mg l-1 cellulase.The enzyme treatment increased the transformation efficiency (TE) 1.6- fold as compared to agroinfection without cellulase. Less pronounced impact on TE exerted 100 mg l-1 acetosyringone, increasing TE 1.3-fold. When tested in soils

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Tissue And Organ Culture

ISSN

0167-6857

e-ISSN

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Volume of the periodical

112

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

321-330

UT code for WoS article

000315277300006

EID of the result in the Scopus database

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