Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48392952%3A_____%2F13%3A%230000264" target="_blank" >RIV/48392952:_____/13:#0000264 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >http://dx.doi.org/10.1007/s11240-012-0239-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-012-0239-1" target="_blank" >10.1007/s11240-012-0239-1</a>

Result language
angličtina
Original language name
Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein
Original language description
The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of ?-domain of mammalian metallothionein 1a (?MT1a) and ?-glucuronidase gus gene under the control of CaMV35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, t
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/1M06030" target="_blank" >1M06030: Functional genomics and proteomics for crop improvement</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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