Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy
Result description
Milk acetone (AC) is important indicator of energy metabolism of cows. The goal was to investigate infrared method MIR?FT in terms of its calibration for milk AC and to develop an usable procedure. Microdiffusion photometric (485 nm; Spekol 11) method with salicylaldehyde as reference (Re) and mid infrared spectroscopy FT (MIR?FT: Lactoscope FT?IR, Delta; MilkoScan FT 6000, M?Sc) as indirect method were used. The acetone addition to milk had no recovery using MIR?FT (Delta). The reference AC set has tohave an acceptable statistics for good MIR?FT calibration (M?Sc). It was 10.1 ? 9.74 at geometric mean 7.26 mg.l?1, variation range from 1.98 to 33.66 mg.l?1. AC correlation between Re and MIR?FT (Delta) was low 0.32 (P > 0.05). Log AC relationship between Re and MIR?FT (M?Sc) was markedly better 0.80 (P < 0.01). The conversion of > 10 mg.l?1 as AC subclinical ketosis limit was: > ?0.80 (feedback 0.158 mmol.l?1 = 9.25 mg.l?1) and > ?1.66, important for ketosis monitoring by M?Sc.
Keywords
cowraw milkketosisacetonebetahydroxybutyratereference samplespectrophotometryinfrared spectroscopycalibrationvalidation
The result's identifiers
Result code in IS VaVaI
Alternative codes found
RIV/49608851:_____/11:#0000484
Result on the web
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy
Original language description
Milk acetone (AC) is important indicator of energy metabolism of cows. The goal was to investigate infrared method MIR?FT in terms of its calibration for milk AC and to develop an usable procedure. Microdiffusion photometric (485 nm; Spekol 11) method with salicylaldehyde as reference (Re) and mid infrared spectroscopy FT (MIR?FT: Lactoscope FT?IR, Delta; MilkoScan FT 6000, M?Sc) as indirect method were used. The acetone addition to milk had no recovery using MIR?FT (Delta). The reference AC set has tohave an acceptable statistics for good MIR?FT calibration (M?Sc). It was 10.1 ? 9.74 at geometric mean 7.26 mg.l?1, variation range from 1.98 to 33.66 mg.l?1. AC correlation between Re and MIR?FT (Delta) was low 0.32 (P > 0.05). Log AC relationship between Re and MIR?FT (M?Sc) was markedly better 0.80 (P < 0.01). The conversion of > 10 mg.l?1 as AC subclinical ketosis limit was: > ?0.80 (feedback 0.158 mmol.l?1 = 9.25 mg.l?1) and > ?1.66, important for ketosis monitoring by M?Sc.
Czech name
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Czech description
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Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Agrobiology
ISSN
1803-4403
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
16
Pages from-to
33-48
UT code for WoS article
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EID of the result in the Scopus database
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Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
GM - Food industry
Year of implementation
2011