Immobilization of Proteolytic Enzymes onto Silica Nanofibers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24510%2F18%3A00007503" target="_blank" >RIV/46747885:24510/18:00007503 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24530/18:00007503

Result on the web

<a href="http://vat.ft.tul.cz/Archive/VaT_2018_4.html" target="_blank" >http://vat.ft.tul.cz/Archive/VaT_2018_4.html</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immobilization of Proteolytic Enzymes onto Silica Nanofibers

Original language description

Even in modern medicine, it is still necessary to remove necrotic tissue from burns in a very painful method – by using surgical instruments. It is possible to replace the surgical method with application of proteolytic enzymes directly to the wound, which removes the necrotic tissue completely painlessly. However, most enzymes are active only for a short time and under the specific conditions. The catalytic activity of enzymes can be increased e.g. by immobilization of enzymes onto the biocompatible silica nanofibers. The nanofibers must be functionalized by suitable reagents to form a ligand between silica nanofibers and amino groups of proteolytic enzymes. In our research, the nanofibers surface was modified by 3-Aminopropyl triethoxysilane firstly, than functionalization by succinic anhydride and N-Hydroxysuccinimide ester was done. As the next step, 7 different proteolytic enzymes were immobilized to the silica nanofibers surface. Proteolytic activity of enzymes was tested under conditions simulated skin burns environment (temperature of 37°C and pH 4.6).

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fibres and textiles (Vlákna a textil)

ISSN

1335-0617

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

4

Country of publishing house

SK - SLOVAKIA

Number of pages

4

Pages from-to

16-19

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85064514920