Typing of MRSA strains using different molecular-genetic methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F11%3A%230000510" target="_blank" >RIV/49608851:_____/11:#0000510 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Typing of MRSA strains using different molecular-genetic methods
Original language description
The aim of our work was to apply different molecular techniques to type MRSA strains, compare acquired results and performance of these methods. The collection of eight MRSA strains from different livestock farms in the Czech Republic was analysed. To type the strains, the MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing, rep-PCR analyse, ribotyping and PFGE (Pulsed-field gel electrophoresis) were applied. The (GTG)5 primer and the GelCompare software were used for the rep-PCR analyse. The ribotyping of the strains was performed on the RiboPrinterTM system, the SmaI restriction endonuclease was applied for the PFGE analyse. The SCCmec typing revealed presence of 4, 4D and 5 SCCmec type, the MLST analyseidentified the ST8 and ST398 type among tested strains. Analysis of spa profiles revealed presence of five different strains. Also the rep-PCR indicated presence of five types of strains, however with distinct distribution. The ribotypin
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů