Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F11%3A%230000511" target="_blank" >RIV/49608851:_____/11:#0000511 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Molecular characterization of MRSA strains isolated from livestock milk and meat in the Czech Republic
Original language description
Aims: The livestock milk and meat industry represent a possible source of MRSA strains, with potential to infect the humans or infiltrate the food chain. The aim of the study was to apply different molecular genetic methods to type acquired MRSA strainsMethods and results: Hundreds of samples of cow, goat and sheep milk, pork meat and farm personnel were collected in two years in the Czech Republic. Eight MRSA strains from different farms were purchased and analysed using MLST (Multi Locus Sequence typing), spa and SCCmec (Staphylococcal Chromosome Cassette mec) typing based on PCR. The (GTG)5 primer was applied for the rep-PCR analyse. The ribotypization of the strains was performed on the RiboPrinterTM system. The individual strains possessed resistance to 3-8 different antibiotics, two isolates carried the gene seb encoding enterotoxin B. The strains belonged to the 4, 4D and 5 SCCmec type; t011, t034 and t064 spa type and ST8, ST398 MLST type. The results of ribotypization reveale
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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