Combined model of bladder detrusor smooth muscle and interstitial cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23640%2F16%3A43929503" target="_blank" >RIV/49777513:23640/16:43929503 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/16:10324245 RIV/49777513:23520/16:43929503

Result on the web

<a href="http://dx.doi.org/10.1177/1535370216655402" target="_blank" >http://dx.doi.org/10.1177/1535370216655402</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1535370216655402" target="_blank" >10.1177/1535370216655402</a>

Result language

angličtina

Original language name

Combined model of bladder detrusor smooth muscle and interstitial cells

Original language description

Understanding the interactions between the detrusor smooth muscle cells and other bladder cell types (e.g. interstitial cells, IC) that may significantly contribute to coordinating and modulating detrusor contractions represents a considerable challenge. Computer modeling could help to elucidate some properties that are difficult to address experimentally; therefore, we developed in silico models of detrusor smooth muscle cell and interstitial cells, coupled through gap junctions. The models include all of the major ion conductances and transporters described in smooth muscle cell and interstitial cells in the literature. The model of normal detrusor muscle (smooth muscle cell and interstitial cells coupled through gap junctions) completely reproduced the experimental results obtained with detrusor strips in the presence of several pharmacological interventions (ryanodine, caffeine, nimodipine), whereas the model of smooth muscle cell alone (without interstitial cells) failed to reproduce the experimental results. Next, a model of overactive bladder, a highly prevalent clinical condition in both men and women with increasing incidence at older ages, was produced by modifying several processes as reported previously: a reduction of Ca2ţ-release through ryanodine receptors and a reduction of Ca2ţ-dependent Kţ-conductance with augmented gap junctional coupling. This model was also able to reproduce the pharmacological modulation of overactive bladder. The results indicate that the non-smooth muscle cells of the detrusor (interstitial cells) contribute significantly to the contractile behavior of bladder detrusor muscle and should not be neglected. The model suggests that reduced Ca2ţ- release through ryanodine receptors and Ca2ţ-dependent Kţ-conductance together with augmented gap junctional coupling might play a major role in overactive bladder pathogenesis.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EXPERIMENTAL BIOLOGY AND MEDICINE

ISSN

1535-3702

e-ISSN

—

Volume of the periodical

241

Issue of the periodical within the volume

16

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

1853-1864

UT code for WoS article

000383439000017

EID of the result in the Scopus database

2-s2.0-84988014662