Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12110%2F14%3A43887830" target="_blank" >RIV/60076658:12110/14:43887830 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3109/09553002.2014.887233" target="_blank" >http://dx.doi.org/10.3109/09553002.2014.887233</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/09553002.2014.887233" target="_blank" >10.3109/09553002.2014.887233</a>

Result language
angličtina
Original language name
Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell
Original language description
PURPOSE Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test thishypothesis. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay andtwo-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C. RESULTS The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediatelybefore or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected. CONCLUSIONS The protective effect of hypothermia observed at the level of cytogenetic d
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)