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Comparison of different dna isolation methods for RAPD, AFLP and PCR-RFLP analyses

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F00%3A00002525" target="_blank" >RIV/60076658:12220/00:00002525 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of different dna isolation methods for RAPD, AFLP and PCR-RFLP analyses

  • Original language description

    The rapid and simple DNA microextraction method is important for a nondestructive, early DNA diagnostic system in plant-breeding programs. We introduce our opportunity with the DNeasy(TM) Plant Mini Kit (QIAGEN), which provides a fast and simple way to isolate total genomic DNA from different plant genera: Brassica, Solanum, Dactylorhiza, Gymnadenia, Gentiana, Gentianella, Orchis, Melampyrum, Dactylis, Lolium, Festuca, Trifolium, Pinus in comparison with other methods of MURRAY and THOMPSON (1980), DELAPORTA et al. (1983) and EDWARDS (1991). We have found DNeasy Plant Mini Kit DNA isolation method suitable for PCR and related methods: RAPD, PCR-RFLP and AFLP.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GE - Plant cultivation

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2000

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Collection of scientific papers, Faculty of agriculture in České Budějovice : series for crop scienc

  • ISSN

    1212-0731

  • e-ISSN

  • Volume of the periodical

    17

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database