Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F01%3A00002839" target="_blank" >RIV/60076658:12220/01:00002839 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.)

Original language description

Esterase isozymes, proteins extracted in 2% glutathione buffer (separated in non denaturing PAGE system) and seed proteins separated in SDS page were used as biochemical markers for evaluation of polymorphysm level in three spelt wheat varieties, three breeders spelt lines (orig. from hybrids. between spelt and common wheat) and reference common wheat variety. Electrophoresis phenotypes and zymograms were evaluated by means of digital image analysis and Nei + Li coufficient if similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of three marker systems showed differences between common wheat variety Brea and spelt varieties and spelt breeders´ lines. Also significant differences between old spelt varieties (Hercule, Altgold and Rouquin) and new spelt breeders´ lines were found.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA303%2F01%2F1380" target="_blank" >GA303/01/1380: Possibilities of spectra products widening for gluten-free diet at coeliac disease: alternative sources, their testing and using</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientia agriculturae bohemica

ISSN

582-2343

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

12

Pages from-to

171-182

UT code for WoS article

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EID of the result in the Scopus database

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