Species-specific PCR primers for detection of Xanthomonas vesicatoria

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F13%3A43883410" target="_blank" >RIV/60076658:12220/13:43883410 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/13:00392154
Result on the web
<a href="http://dx.doi.org/10.1016/j.cropro.2012.08.008" target="_blank" >http://dx.doi.org/10.1016/j.cropro.2012.08.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cropro.2012.08.008" target="_blank" >10.1016/j.cropro.2012.08.008</a>

Result language
angličtina
Original language name
Species-specific PCR primers for detection of Xanthomonas vesicatoria
Original language description
This study present the development of a PCR method for specific detection of Xanthomonas vesicatoria, casual agent of bacterial spot of tomato and pepper. Primer pair XV1F and XV1R was designed on partial DNA sequence of the catalytic subunit of the ATPsynthase gene atpD and tested using a collection of various bacterial pathogens mainly for tomato and pepper. After optimization of the PCR conditions, the 365 bp long fragment was only produced in X. vesicatoria samples. Therefore, the primers and protocol allow rapid and species-specific detection of X. vesicatoria.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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