Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F15%3A43887835" target="_blank" >RIV/60076658:12220/15:43887835 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/15:00443160
Result on the web
<a href="http://dx.doi.org/10.1007/s10658-014-0554-y" target="_blank" >http://dx.doi.org/10.1007/s10658-014-0554-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10658-014-0554-y" target="_blank" >10.1007/s10658-014-0554-y</a>

Result language
angličtina
Original language name
Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria
Original language description
We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia andcentral and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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