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Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F19%3A43899722" target="_blank" >RIV/60076658:12220/19:43899722 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43510/20:43916477

  • Result on the web

    <a href="https://doi.org/10.1016/j.cropro.2019.104978" target="_blank" >https://doi.org/10.1016/j.cropro.2019.104978</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.cropro.2019.104978" target="_blank" >10.1016/j.cropro.2019.104978</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria

  • Original language description

    New species-specific PCR primers for detection of bacterium Xanthomonas euvesicatoria, the causal agent of bacterial spot of tomato and pepper, were designed in this work. Developed PCR primer pair ZnDoF/ZnDoR partially targets the coding sequence of Zn-dependent oxidoreductase gene. The primer pair was tested on various bacterial strains including bacteria from the bacterial spot-causing xanthomonads (BSX) group. Designed primers showed to be species-specific for the samples containing DNA of X. euvesicatoria. Specificity of the designed primers was also compared with the primer pairs already published. Subsequently, sensitivity of ZnDoF/ZnDoR primers was determined by ten-fold dilution of standardized sample. Detection capability of developed primers was also investigated on artificially infected pepper plants and seeds collected from bacterial spot-like peppers. Primers proved to be species-specific for in planta and in seed detection as well as to distinguish X. euvesicatoria from other bacterial strains used in this assay.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Crop protection

  • ISSN

    0261-2194

  • e-ISSN

  • Volume of the periodical

    127

  • Issue of the periodical within the volume

    leden 2020

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    5

  • Pages from-to

    1-5

  • UT code for WoS article

    000498749700027

  • EID of the result in the Scopus database

    2-s2.0-85072948364