Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F20%3A43916477" target="_blank" >RIV/62156489:43510/20:43916477 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12220/19:43899722
Result on the web
<a href="https://doi.org/10.1016/j.cropro.2019.104978" target="_blank" >https://doi.org/10.1016/j.cropro.2019.104978</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cropro.2019.104978" target="_blank" >10.1016/j.cropro.2019.104978</a>

Result language
angličtina
Original language name
Species-specific PCR primers for the detection of poorly distinguishable Xanthomonas euvesicatoria
Original language description
New species-specific PCR primers for detection of bacterium Xanthomonas euvesicatoria, the causal agent of bacterial spot of tomato and pepper, were designed in this work. Developed PCR primer pair ZnDoF/ZnDoR partially targets the coding sequence of Zn-dependent oxidoreductase gene. The primer pair was tested on various bacterial strains including bacteria from the bacterial spot-causing xanthomonads (BSX) group. Designed primers showed to be species-specific for the samples containing DNA of X. euvesicatoria. Specificity of the designed primers was also compared with the primer pairs already published. Subsequently, sensitivity of ZnDoF/ZnDoR primers was determined by ten-fold dilution of standardized sample. Detection capability of developed primers was also investigated on artificially infected pepper plants and seeds collected from bacterial spot-like peppers. Primers proved to be species-specific for in planta and in seed detection as well as to distinguish X. euvesicatoria from other bacterial strains used in this assay.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multidisciplinary research to increase application potential of nanomaterials in agricultural practice</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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