Preparation of recombinant coat protein of Prunus necrotic ringspot virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F01%3A00003521" target="_blank" >RIV/60076658:12310/01:00003521 - isvavai.cz</a>

Alternative codes found

RIV/60077352:_____/01:63010039

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Preparation of recombinant coat protein of Prunus necrotic ringspot virus

Original language description

The coat protein (CP) gene of prunus necrotic ringspot virus (PNRSV) was cloned into pET16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins eas used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F99%2F0437" target="_blank" >GA522/99/0437: Preparation of recombinant plant virus coat protein in bacteria and yeast</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Virologica

ISSN

0001-723X

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

3

Pages from-to

61-63

UT code for WoS article

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EID of the result in the Scopus database

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