Characterization of two closely related alpha-amylase paralogs in the bark beetle, Ips typographus (L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F11%3A43882400" target="_blank" >RIV/60076658:12310/11:43882400 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/arch.20433" target="_blank" >http://dx.doi.org/10.1002/arch.20433</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/arch.20433" target="_blank" >10.1002/arch.20433</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of two closely related alpha-amylase paralogs in the bark beetle, Ips typographus (L.)
Original language description
Ips typographus (L.), the eight-spined spruce bark beetle, causes severe damage throughout Eurasian spruce forests and suitable nuclear markers are needed in order to study its population structure on a genetic level. Two closely related genes encoding alpha-amylase in I. typographus were characterized and named AmyA and AmyB. Both alpha-amylase paralogs consisted of six exons and five introns. AmyA encodes a polypeptide of 483 amino acids, whereas AmyB has two alternative transcripts encoding polypeptides of 483 and 370 amino acids. The expression levels of both genes were high during larval stage and adulthood. The AmyB transcripts were absent in the pupal stage. A modification of the allozyme staining method allowed us to detect two clusters of bands on the electrophoretic gel that may correspond to the two alpha-amylase genes. There was a correlation between the lack of AmyB expression in pupa and the absence of the fast migrating isozyme cluster at this stage, suggesting that the
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Insect Biochemistry and Physiology
ISSN
0739-4462
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
179-198
UT code for WoS article
000295287900002
EID of the result in the Scopus database
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