Current standard assays using artificial substrates overestimate phosphodiesterase activity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F13%3A43885615" target="_blank" >RIV/60076658:12310/13:43885615 - isvavai.cz</a>
Result on the web
<a href="http://ac.els-cdn.com/S0038071712000600/1-s2.0-S0038071712000600-main.pdf?_tid=64502ab6-840b-11e3-9277-00000aab0f6c&acdnat=1390467201_dd136a4b5fbbfc9681510c516fb23042" target="_blank" >http://ac.els-cdn.com/S0038071712000600/1-s2.0-S0038071712000600-main.pdf?_tid=64502ab6-840b-11e3-9277-00000aab0f6c&acdnat=1390467201_dd136a4b5fbbfc9681510c516fb23042</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.soilbio.2012.02.008" target="_blank" >10.1016/j.soilbio.2012.02.008</a>

Result language
angličtina
Original language name
Current standard assays using artificial substrates overestimate phosphodiesterase activity
Original language description
Phosphodiesterases (PDE) are important enzymes in environments with significant proportion of phosphodiesters. The breakdown of phosphodiesters is a two step process, initiated by PDE hydrolyzing an ester-P bond on the diester molecule, breaking it intoan organic moiety and a phosphomonoester. In the second step, the phosphomonoester is further hydrolyzed by phosphomonoesterase (PME) into the second organic moiety and free phosphate, P-i. The methods to assess extracellular PDE activities commonly usethe substrate analogues bis-(p-nitrophenyl) phosphate (bis-pNPP) or bis-(4-methylumbelliferyl) phosphate (bis-MUFP), measure the resulting concentration of p-nitrophenol (pNP) or 4-methylumbelliferon (MUF), and assume the contribution from phosphomonoester hydrolysis by PME to be insignificant. To verify this, we measured concentrations of MUF and P-i at the end of the 30- and 60-min incubation of wetland plant root segments with a phosphodiester substrate. We found that the hydrolysis w
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DF - Pedology
OECD FORD branch
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Project
<a href="/en/project/ME%20912" target="_blank" >ME 912: Linking ecosystem processes and community structure in tropical marshes: the role of macrophytes and microbial communities</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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