Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43887570" target="_blank" >RIV/60076658:12310/14:43887570 - isvavai.cz</a>

Result on the web

<a href="http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0113873" target="_blank" >http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0113873</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0113873" target="_blank" >10.1371/journal.pone.0113873</a>

Result language

angličtina

Original language name

Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight

Original language description

ArcLight, a genetically encoded fluorescent protein voltage probe with a large Delta F/Delta V, is a fusion between the voltage sensing domain of the Ciona instestinalis voltage sensitive phosphatase and super ecliptic pHluorin carrying a single mutation(A227D in the fluorescent protein). Without this mutation the probe produces only a very small change in fluorescence in response to voltage deflections (similar to 1%). The large signal afforded by this mutation allows optical detection of action potentials and sub-threshold electrical events in single-trials in vitro and in vivo. However, it is unclear how this single mutation produces a probe with such a large modulation of its fluorescence output with changes in membrane potential. In this study, we identified which residues in super ecliptic pHluorin (vs eGFP) are critical for the ArcLight response, as a similarly constructed probe based on eGFP also exhibits large response amplitude if it carries these critical residues. We found

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

21

Pages from-to

—

UT code for WoS article

000346766900077

EID of the result in the Scopus database

—