MLL2 conveys transcription-independent H3K4 trimethylation in oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897254" target="_blank" >RIV/60076658:12310/18:43897254 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/18:00488581

Result on the web

<a href="https://www.nature.com/articles/s41594-017-0013-5" target="_blank" >https://www.nature.com/articles/s41594-017-0013-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41594-017-0013-5" target="_blank" >10.1038/s41594-017-0013-5</a>

Result language

angličtina

Original language name

MLL2 conveys transcription-independent H3K4 trimethylation in oocytes

Original language description

Histone 3 K4 trimethylation (depositing H3K4me3 marks) is typically associated with active promoters yet paradoxically occurs at untranscribed domains. Research to delineate the mechanisms of targeting H3K4 methyltransferases is ongoing. The oocyte provides an attractive system to investigate these mechanisms, because extensive H3K4me3 acquisition occurs in nondividing cells. We developed low-input chromatin immunoprecipitation to interrogate H3K4me3, H3K27ac and H3K27me3 marks throughout oogenesis. In nongrowing oocytes, H3K4me3 was restricted to active promoters, but as oogenesis progressed, H3K4me3 accumulated in a transcription-independent manner and was targeted to intergenic regions, putative enhancers and silent H3K27me3-marked promoters. Ablation of the H3K4 methyltransferase gene Mll2 resulted in loss of transcription-independent H3K4 trimethylation but had limited effects on transcription-coupled H3K4 trimethylation or gene expression. Deletion of Dnmt3a and Dnmt3b showed that DNA methylation protects regions from acquiring H3K4me3. Our findings reveal two independent mechanisms of targeting H3K4me3 to genomic elements, with MLL2 recruited to unmethylated CpG-rich regions independently of transcription.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Structural &amp; Molecular Biology

ISSN

1545-9993

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

73-84

UT code for WoS article

000423547700011

EID of the result in the Scopus database

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