Trypanosomal mitochondrial intermediate peptidase does not behave as a classical mitochondrial processing peptidase

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897392" target="_blank" >RIV/60076658:12310/18:43897392 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498793 RIV/00216208:11310/18:10376227
Result on the web
<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0196474" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0196474</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0196474" target="_blank" >10.1371/journal.pone.0196474</a>

Result language
angličtina
Original language name
Trypanosomal mitochondrial intermediate peptidase does not behave as a classical mitochondrial processing peptidase
Original language description
Upon their translocation into the mitochondrial matrix, the N-terminal pre-sequence of nuclear-encoded proteins undergoes cleavage by mitochondrial processing peptidases. Some proteins require more than a single processing step, which involves several peptidases. Down-regulation of the putative Trypanosoma brucei mitochondrial intermediate peptidase (MIP) homolog by RNAi renders the cells unable to grow after 48 hours of induction. Ablation of MIP results in the accumulation of the precursor of the trypanosomatid-specific trCOIV protein, the largest nuclear-encoded subunit of the cytochrome c oxidase complex in this flagellate. However, the trCOIV precursor of the same size accumulates also in trypanosomes in which either alpha or beta subunits of the mitochondrial processing peptidase (MPP) have been depleted. Using a chimeric protein that consists of the N-terminal sequence of a putative subunit of respiratory complex I fused to a yellow fluorescent protein, we assessed the accumulation of the precursor protein in trypanosomes, in which RNAi was induced against the alpha or beta subunits of MPP or MIP. The observed accumulation of precursors indicates MIP depletion affects the activity of the cannonical MPP, or at least one of its subunits.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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