The innovation of cryo-SEM freeze-fracturing methodology demonstrated on high pressure frozen biofilm
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897418" target="_blank" >RIV/60076658:12310/18:43897418 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00491213 RIV/68081731:_____/18:00491213 RIV/00216224:14110/18:00104166 RIV/00159816:_____/18:00068743
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0968432818300209?token=A5A4808408926CF767C4AB7CA900E48333FEC796F0EB9F7A014D3109DFF923AC5C5A4625C149BB780C41AA53096E99EA" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0968432818300209?token=A5A4808408926CF767C4AB7CA900E48333FEC796F0EB9F7A014D3109DFF923AC5C5A4625C149BB780C41AA53096E99EA</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micron.2018.04.006" target="_blank" >10.1016/j.micron.2018.04.006</a>
Alternative languages
Result language
angličtina
Original language name
The innovation of cryo-SEM freeze-fracturing methodology demonstrated on high pressure frozen biofilm
Original language description
In this study we present an innovative method for the preparation of fully hydrated samples of microbial biofilms of cultures Staphylococcus epidermidis, Candida parapsilosis and Candida albicans. Cryo-scanning electron micro-scopy (cryo-SEM) and high-pressure freezing (HPF) rank among cutting edge techniques in the electron microscopy of hydrated samples such as biofilms. However, the combination of these techniques is not always easily applicable. Therefore, we present a method of combining high-pressure freezing using EM PACT2 (Leica Microsystems), which fixes hydrated samples on small sapphire discs, with a high resolution SEM equipped with the widely used cryo-preparation system ALTO 2500 (Gatan). Using a holder developed in house, a freeze fracturing technique was applied to image and investigate microbial cultures cultivated on the sapphire discs. In our experiments, we focused on the ultrastructure of the extracellular matrix produced during cultivation and the relationships among microbial cells in the biofilm. The main goal of our investigations was the detailed visualization of areas of the biofilm where the microbial cells adhere to the substrate/surface. We show the feasibility of this technique, which is clearly demonstrated in experiments with various freeze-etching times.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20201 - Electrical and electronic engineering
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Micron
ISSN
0968-4328
e-ISSN
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Volume of the periodical
110
Issue of the periodical within the volume
JUL 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
28-35
UT code for WoS article
000435048000004
EID of the result in the Scopus database
2-s2.0-85046133186