Cryo-SEM of Perpendicular Cross Freeze-Fractures Through a High-Pressure-frozen Biofilm

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00436621" target="_blank" >RIV/68081731:_____/14:00436621 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26210/14:PU110182
Result on the web
<a href="http://dx.doi.org/10.1017/S1431927614007892" target="_blank" >http://dx.doi.org/10.1017/S1431927614007892</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1431927614007892" target="_blank" >10.1017/S1431927614007892</a>

Result language
angličtina
Original language name
Cryo-SEM of Perpendicular Cross Freeze-Fractures Through a High-Pressure-frozen Biofilm
Original language description
The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples. However, their combination is not always easily applicable. Here, we present a way ofcombining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolution SEM JEOL 7401F equipped with the cryo-attachment ALTO 2500 (GATAN). The freeze-fracturing technique is used here for investigation of yeast cultures cultivated on the sapphire discs, where we focus on the formation of the extracellular matrix produced during the cultivation.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)