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Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899503" target="_blank" >RIV/60076658:12310/19:43899503 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/19:10397028

  • Result on the web

    <a href="https://reader.elsevier.com/reader/sd/pii/S1386142519300137?token=A7C4FBCD5BBB8DB090DDD391D3BC16EE32BFFCB4B6F52D1C5B11A3D974EB9983E27C702FFCFA3C531C2B5E96568653DA" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S1386142519300137?token=A7C4FBCD5BBB8DB090DDD391D3BC16EE32BFFCB4B6F52D1C5B11A3D974EB9983E27C702FFCFA3C531C2B5E96568653DA</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.saa.2019.01.013" target="_blank" >10.1016/j.saa.2019.01.013</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography

  • Original language description

    We tested the potential of Raman microspectroscopy to determine carotenoid pigments - both primary (lutein, beta-carotene) and secondary (astaxanthin) carotenoids - in the different species and life-cycle stages of snow algae from the order Chlamydomonadales (Chlorophyta). We compared the performance of Raman spectrometry to a reference method of biological pigment analysis, high-performance liquid chromatography (HPLC). The three main carotenoid Raman bands of the astaxanthin-rich red cysts were located at 1520, 1156 and 1006 cm(-1). The shifts (orange aplanozygotes and green motile cells with flagella) in the position of the v(1) (C-C) Raman band of the polyenic chain is consistent with the expected changes in the ratios of the various carotenoid pigments. Flagellated green cells commonly contain lutein as a major carotenoid, together with minor amounts of beta-carotene and varying amounts of antheraxanthin, violaxanthin and neoxanthin. Aplanozygotes contain mixtures of both primary and secondary carotenoids. In most cases, the v(1)(C-C) band is an overlapping set of bands, which is due to the signal of all carotenoid pigments in the sample, and a deconvolution along with the band position shifts (mainly v(1)) could be used to characterize the mixture of carotenoids. However, the ability of Raman spectroscopy to discriminate between structurally slightly differing carotenoid pigments or several carotenoids in an admixture in an unknown biological system remains limited. (C) 2019 Elsevier B.V. All rights reserved.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10403 - Physical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy

  • ISSN

    1386-1425

  • e-ISSN

  • Volume of the periodical

    212

  • Issue of the periodical within the volume

    APR 5 2019

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    10

  • Pages from-to

    262-271

  • UT code for WoS article

    000471307100033

  • EID of the result in the Scopus database

    2-s2.0-85059863353