Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00506195" target="_blank" >RIV/61388971:_____/19:00506195 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1386142519300137?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1386142519300137?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.saa.2019.01.013" target="_blank" >10.1016/j.saa.2019.01.013</a>
Alternative languages
Result language
angličtina
Original language name
Analyzing carotenoids of snow algae by Raman microspectroscopy and high-performance liquid chromatography
Original language description
We tested the potential of Raman microspectroscopy to determine carotenoid pigments - both primary (lutein, beta-carotene) and secondary (astaxanthin) carotenoids - in the different species and life-cycle stages of snow algae from the order Chlamydomonadales (Chlorophyta). We compared the performance of Raman spectrometry to a reference method of biological pigment analysis, high-performance liquid chromatography (HPLC). The three main carotenoid Raman bands of the astaxanthin-rich red cysts were located at 1520, 1156 and 1006 cm(-1). The shifts (orange aplanozygotes and green motile cells with flagella) in the position of the v(1) (C-C) Raman band of the polyenic chain is consistent with the expected changes in the ratios of the various carotenoid pigments. Flagellated green cells commonly contain lutein as a major carotenoid, together with minor amounts of beta-carotene and varying amounts of antheraxanthin, violaxanthin and neoxanthin. Aplanozygotes contain mixtures of both primary and secondary carotenoids. In most cases, the v(1)(C-C) band is an overlapping set of bands, which is due to the signal of all carotenoid pigments in the sample, and a deconvolution along with the band position shifts (mainly v(1)) could be used to characterize the mixture of carotenoids. However, the ability of Raman spectroscopy to discriminate between structurally slightly differing carotenoid pigments or several carotenoids in an admixture in an unknown biological system remains limited.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
ISSN
1873-3557
e-ISSN
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Volume of the periodical
212
Issue of the periodical within the volume
APR 5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
262-271
UT code for WoS article
000471307100033
EID of the result in the Scopus database
2-s2.0-85059863353