Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901347" target="_blank" >RIV/60076658:12310/20:43901347 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/20:00531531
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acschembio.0c00178" target="_blank" >https://pubs.acs.org/doi/10.1021/acschembio.0c00178</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acschembio.0c00178" target="_blank" >10.1021/acschembio.0c00178</a>
Alternative languages
Result language
angličtina
Original language name
Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation
Original language description
Dinucleoside polyphosphates (Np(n)Ns) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5' RNA caps in Escherichia coll. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed Np(n)Ns to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of Ap(n)Gs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LL1603" target="_blank" >LL1603: Viral RNA modifications – Essential steps in Chemical Evolution of Protein Cofactors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Chemical Biology
ISSN
1554-8929
e-ISSN
—
Volume of the periodical
15
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1765-1772
UT code for WoS article
000551550000006
EID of the result in the Scopus database
2-s2.0-85088279012