Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43879713" target="_blank" >RIV/60076658:12520/11:43879713 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1439-0426.2011.01866.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0426.2011.01866.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1439-0426.2011.01866.x" target="_blank" >10.1111/j.1439-0426.2011.01866.x</a>

Result language
angličtina
Original language name
Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
Original language description
The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of Acipenser ruthenus sperm. Four different cryoprotectans [dimethylsulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30mM sucrose, 1 mM KCl, 25 mM Tris-HCl pH 8.5) were used for the cryopreservation of sperm from five males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post-thawing motility was observed in sperm samples cryopreserved with 10% MET (46+/-19%), 10% DMA (47+/-18%) and 10% DMSO (45+/-7%). Fertilization/hatching rate in the control was 69+/-9% and 61+/-8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32+/-17%) and 5% DMA (23+/-3%) when compared to 10% DMA (9+/-5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon sperma
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GL - Fishery
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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