Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004203" target="_blank" >RIV/60076658:_____/02:00004203 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation
Original language description
Gene resources include original Czech fish strains as a part of our national culture heritage (7 strains of common carp and 2 strains of European catfish). In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and European catfish. A sperm freezing procedure was developed, providing good motility of spermatozoa, fertilization and hatching rates in each of the species. Common carp sperm was collected in plastic containers (1/10 - 1/30 volume of sperm andair) and stored under aerobic condition at 4 oC for 1-3 hours. Sperm was diluted 1:5 in a Kurokura extender (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3) and equilibrated for 40 minutes at 4 oC. Diluted sperm was transferred to 2 ml cryotube sand 10 % of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 oC to -9 oC at a rate of 4 oC . min-1, then from -11 oC to -80 oC at a rate of 11 oC .min-1, hold for
Czech name
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Czech description
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Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
International Conference Joined Meeting of the IIR with the Society for Low Temperature Biology"CRYOPRESERVATION AND SAFE KEEPING OF CELLS AND TISSUES
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
1
Publisher name
Society for Low Temperature Biology
Place of publication
Hradec Králové
Event location
Hradec Králové
Event date
May 13, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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