Tolbutamide hydroxylation by hepatic microsomes from Atlantic salmon (Salmo salar L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883439" target="_blank" >RIV/60076658:12520/12:43883439 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11033-012-1512-4" target="_blank" >http://dx.doi.org/10.1007/s11033-012-1512-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11033-012-1512-4" target="_blank" >10.1007/s11033-012-1512-4</a>
Alternative languages
Result language
angličtina
Original language name
Tolbutamide hydroxylation by hepatic microsomes from Atlantic salmon (Salmo salar L.)
Original language description
Metabolic transformations of two substrates for human cytochrome P450 (CYP450) 2C9, tolbutamide and diclofenac, were investigated in hepatic microsomes from Atlantic salmon (Salmo salar L.). Tolbutamide hydroxylation followed Michaelis-Menten kinetics. Mean apparent Michaelis-Menten constant (K-m) and maximum reaction velocity (V-max) values for 4-hydroxytolbutamide (TBOH) formation were 0.09 +/- A 0.031 mM and 49.5 +/- A 6.03 pmol/min/mg, respectively. Addition of sulfaphenazole, an inhibitor for mammalian CYP2C9, in a range from 1 to 200 mu M decreased formation of TBOH in a concentration-dependent manner, but not to 50%. Neither fluconazole, an inhibitor of human CYP2C9, nor ketoconazole, inhibitor of CYP1A and CYP3A in fish, affected TBOH formation. In contrast ellipticine, an inhibitor of CYP1A in fish inhibited TBOH formation with the IC50 value of 12.1 mu M. The rate of TBOH formation was competitively inhibited by 100 mu M of sesamin in the incubations, but the degree of inhibi
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology Reports
ISSN
0301-4851
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
6867-6873
UT code for WoS article
000303351900054
EID of the result in the Scopus database
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